Locked nucleic acid real-time polymerase chain reaction method identifying two polymorphisms of hepatitis B virus genotype C2 infections, rt269L and rt269IArticle Published on 2023-03-212023-06-12 Journal: World journal of gastroenterology [Category] B형 간염, [키워드] Chronic Hepatitis B Genotype C2 Hepatitis B virus Locked nucleic acid-real time-polymerase chain reaction polymerase rt269. [DOI] 10.3748/wjg.v29.i11.1721 PMC 바로가기
Multiple ligation-Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2Article Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 치료기술, 치료제, [키워드] acute respiratory syndrome amplification Analysis Bacterial colorimetric detection Colorimetric sensing conditions coronavirus detection system diagnosing disease Diseases effective Genome isothermal isothermal amplification limit of detection Multiple PK-probe point-of-care polymerase probe Pyrophosphate recombinase polymerase amplification Recombinase polymerase amplification (RPA) RNA robust SARS-CoV-2 SARS-CoV-2. selective sensitivity [DOI] 10.1016/j.talanta.2022.123835 PMC 바로가기
Evaluation of indirect sequence-specific magneto-extraction-aided LAMP for fluorescence and electrochemical SARS-CoV-2 nucleic acid detectionArticle Published on 2023-01-152022-11-15 Journal: Talanta [Category] SARS, 변종, 치료기술, 치료제, [키워드] amplification approach circumvent clinically Disadvantages Electrochemical LAMP Host in silico inhibitor investigated isothermal isothermal amplification Magnetic sequence-specific target enrichment molecular NAAT nucleic acid Nucleic acid detection PCR polymerase probe qRT-PCR Quantitative Quantitative real-time LAMP real-time reverse transcriptase restrict SARS-CoV-2 SARS-CoV-2 nucleic acid SARS-CoV-2 variant SARS-CoV-2. sensitivity sequence serum turn [DOI] 10.1016/j.talanta.2022.123809 PMC 바로가기
Nanozyme-strip for rapid and ultrasensitive nucleic acid detection of SARS-CoV-2Article Published on 2022-12-012022-11-15 Journal: Biosensors & bioelectronics [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome amplification amplify catalytic clinical sample coronavirus coronavirus disease COVID-19 develop Diagnosis gold standard highlighting HPV-16 limit of detection Nanozyme strips nontoxic nucleic acid nucleic acid amplification Nucleic acid detection pandemic POCT point-of-care point-of-care testing polymerase REcombinase recombinase polymerase amplification reliability RPA RT-PCR SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. subsequent TMB unique viral infections virus [DOI] 10.1016/j.bios.2022.114739 PMC 바로가기
Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replicationArticle Published on 2022-10-142022-11-16 Journal: Life Science Alliance [Category] COVID19(2023년), SARS, 변종, [키워드] Analysis association Calu-3 cell cellular Comorbidity components COVID-19 demonstrated effective eQTL examined EXOSC2 expression form Genome-wide association study higher risk host protein host-virus interactions human RNA identify impede increased expression interact Interaction loci New nonsense mutation polymerase PROTECT Protein protein expression reduced risk loci RNA RNA polymerase Safe SARS-CoV-2 proteins SARS-CoV-2 replication SARS-CoV-2 viral infection the SARS-CoV-2 therapeutic target Treatment valuable resource viability Viral RNA with COVID-19 [DOI] 10.26508/lsa.202201449 PMC 바로가기
Automated, portable, and high-throughput fluorescence analyzer (APHF-analyzer) and lateral flow strip based on CRISPR/Cas13a for sensitive and visual detection of SARS-CoV-2SARS-CoV-2의 감도 및 시각적 검출을 위한 CRISPR/Cas13a 기반의 자동화된 휴대용 고처리량 형광 분석기(APHF 분석기) 및 측면 흐름 스트립Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 치료기술, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 addressed amplification automated biosensor Care Contamination coronavirus COVID-19 CRISPR CRISPR/Cas13a detect Detection limit develop globe Health Lateral lateral flow strip MOST On-site detection ORF1ab gene POCT Point point of care Point of care testing (POCT) polymerase provided recombinase polymerase amplification Recombinase polymerase amplification (RPA) RPA S gene SARS-CoV-2 SARS-CoV-2 detection SARS-CoV-2. severe acute respiratory syndrome Coronavirus specificity Visualization [DOI] 10.1016/j.talanta.2022.123594 PMC 바로가기 [Article Type] Article
Laboratory and field evaluation of the STANDARD Q and Panbio™ SARS-CoV-2 antigen rapid test in Namibia using nasopharyngeal samplesArticle Published on 2022-09-272022-11-15 Journal: PLoS ONE [Category] SARS, 변종, 진단, 치료기술, [키워드] Ag-RDT Ag-RDTs alternative Antigen Asymptomatic Coronavirus disease 2019 country COVID-19 COVID-19 infection detect diagnostics evaluated Laboratory nasopharyngeal sample nasopharyngeal swabs new SARS-CoV-2 Participants polymerase Rapid test resource resources RT-PCR SARS-CoV-2 SARS-CoV-2 antigen SARS-CoV-2 testing sensitivity specificity symptomatic transcriptase Transmission variants of concern was reduced [DOI] 10.1371/journal.pone.0269329 PMC 바로가기
Stenoparib, an inhibitor of cellular poly (ADP-ribose) polymerases (PARPs), blocks in vitro replication of SARS-CoV-2 variantsArticle Published on 2022-09-142022-11-16 Journal: PLoS ONE [Category] SARS, 변종, [키워드] 2X-121 50% effective concentration ADP-ribose affecting Alpha antiviral drug B.1.1.7 B.1.351 B.1.617.2 block cellular Combination EC50 Effect estimate experiment greater Host in vitro inhibit inhibitor mechanism P.1 PARP PARPs pathway polymerase Remdesivir replication of SARS-CoV-2 SARS-CoV-2 stenoparib variant viral replication wild type [DOI] 10.1371/journal.pone.0272916 PMC 바로가기
Modeling the Enzymatic Mechanism of the SARS-CoV-2 RNA-Dependent RNA Polymerase by DFT/MM-MD: An Unusual Active Site Leading to High Replication RatesArticle Published on 2022-09-122022-11-15 Journal: Journal of Chemical Information and Modeling [Category] SARS, 유전자 메커니즘, 치료제, [키워드] absence Activation Antiviral assist cellular compensated computation COVID-19 diffusion duplication Efficiency Enzymes highlight histidine Inclusion infecting Infection lack leading less Lower lysine mechanism modeling nucleotide Pandemics performed polymerase polymerases Rate reactive reactivity Replication replication of SARS-CoV-2 RNA RNA genome RNA viruses RNA-dependent RNA polymerase SARS-CoV-2 genome Site subsequent the SARS-CoV-2 viral genome Viral RNA virus [DOI] 10.1021/acs.jcim.2c00802 PMC 바로가기
In silico prediction of SARS-CoV-2 main protease and polymerase inhibitors: 3D-Pharmacophore modellingSARS-CoV-2 주요 프로테아제 및 폴리머라제 억제제의 인 실리코 예측: 3D-Pharmacophore 모델링Article Published on 2022-09-012022-09-11 Journal: Journal of biomolecular structure & dynamics [Category] SARS, 치료제, [키워드] 3CLpro active site acute respiratory syndrome acute respiratory syndrome coronavirus addition affinity applied approach approved Atazanavir AutoDock binding affinity binding pocket candidate caused chymotrypsin coronavirus COVID-19 determine docking docking study drug effective enzyme hepatitis C hepatitis C virus HIV protease inhibitor identify in silico inhibitors inspiration lowest methodology outbreak overcome pandemic pharmacophore modelling polymerase predict Prevent protease protease 3Clpro. protocol RdRP RNA-dependent RNA polymerase SARS-CoV-2 SARS-CoV-2 main protease social distancing stable complex Structure structures suggested Tegobuvir tipranavir Transmission Treatment Vaccine virus [DOI] 10.1080/07391102.2021.1886991 PMC 바로가기 [Article Type] Article